Hersteller |
Boster
|
Kategorie |
|
Typ |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat |
Isotype |
IgG |
Format |
Lyophilized |
Applikationen |
WB, IHC, ELISA |
Menge |
100 ug/vial |
Host |
Rabbit |
ArtNr |
BOS-PB9944 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Rabbit Polyclonal Antibodies |
Manufacturers Research Category |
Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction |
Short Description |
Boster Bio Anti-FGF1 Antibody Picoband™ catalog # PB9944. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-FGF1 Antibody Picoband™ catalog # PB9944. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It also acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. |
Gene Name |
FGF1 |
Application Details |
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat ELISA , 0.1-0.5μg/ml, Human, - Western blot, 0.1-0.5μg/ml, Rat, Human |
Application Notes |
Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
Clonality |
Polyclonal |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Contents |
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen |
E. coli-derived human FGF1 recombinant protein (Position: F16-D155). Human FGF1 shares 96.4% amino acid (aa) sequence identity with both mouse and rat FGF1. |
Purification |
Immunogen affinity purified. |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene Full Name |
Fibroblast growth factor 1 |
Molecular Weight |
17460 MW |
Protein Function |
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.. |
Subcellular Localization |
Secreted. Cytoplasm. Cytoplasm, cell cortex. Cytoplasm, cytosol. Nucleus. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu (2+) ions and S100A13. Secreted in a complex with SYT1 (By similarity). Binding of exogenous FGF1 to FGFR facilitates endocytosis followed by translocation of FGF1 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as LRRC59.. |
Tissue Specificity |
Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle.. |
Protein Name |
Fibroblast growth factor 1 |
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