Anti-Nestin Antibody Picoband®

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

177439 MW

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml

Nestin

Nestin  is a  type VI  intermediate filament  (IF) protein  that in humans is encoded by the NES  gene. This gene is mapped to 1q23.1. Distribution and expression of nestin in mitotically active cells suggests it plays a role in regulation of the assembly and disassembly of intermediate filaments, which, together with other structural proteins, participate in remodeling of the cell. The role of nestin in dynamic cells, particularly structural organization of the cell, appears strictly regulated by phosphorylation, especially its integration into heterogeneous intermediate filaments together with vimentin or α -internexin. Furthermore, nestin expression has been extensively used as a marker for central nervous system (CNS) progenitor cells in different contexts, based on observations indicating a correlation between nestin expression and this cell type in vivo.

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Angiogenesis, Cancer, Cardiovascular, Cell Adhesion Proteins, Cell Type Marker, Developmental Biology, Intermediate Filaments, Intracellular, Invasion/Microenvironment, Lineage Markers, Lineage Specification, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Stem Cells, Tumor Antigens, Tumor Biomarkers

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells (By similarity).

Boster Bio Anti-Nestin Antibody Picoband® catalog # PB9874. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

No cross-reactivity with other proteins.