Anti-PBP/PEBP1 Antibody Picoband®

Primary Antibodies

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

20830 MW

Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, Rat, By Heat
Western blot, 0.1-0.5?g/ml, Mouse, Rat

Phosphatidylethanolamine-binding protein 1

PEBP1 (Phosphatidylethanolamine-binding protein 1), also called PBP, RKIP, inhibits the phosphorylation and activation of MEK by RAF1. PEBP1 is identical to the phosphatidylethanolamine-binding protein (PBP) with a relative molecular mass of 23 kD. The PEBP1 gene is mapped on 12q24.23. PEBP1 coimmunoprecipitates with RAF1 and MEK from cell lysates and colocalizes with RAF1 when examined by confocal microscopy. PEBP1 overexpression interferes with the activation of MEK and ERK, induction of AP1-dependent reporter genes, and transformation elicited by an oncogenically activated RAF1 kinase. PEBP1 expression was rapidly upregulated during induction of chemotherapy-triggered apoptosis in human prostate and breast cancer cell lines, and maximal RKIP expression correlated perfectly with the onset of apoptosis by Chatterjee et al (2004). RKIP depletion decreased the mitotic index, the number of metaphase cells, traversal times from nuclear envelope breakdown to anaphase, and an override of mitotic checkpoints induced by spindle poisons.

Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.

Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.

Cancer, Cell Biology, Cell Cycle, Cell Division, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Neuropeptides, Neuroscience, Neurotransmitter, Oncoproteins/Suppressors, Spindle, Tumor Suppressors

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. Inhibits the kinase activity of RAF1 by inhibiting its activation and by dissociating the RAF1/MEK complex and acting as a competitive inhibitor of MEK phosphorylation (By similarity).

Boster Bio Anti-PBP/PEBP1 Antibody catalog # PA1713. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Belongs to the MCM family.