ArtNr |
BOS-A00360-2 |
Hersteller |
Boster
|
Menge |
100 ug/vial |
Kategorie |
|
Typ |
Antibody Polyclonal |
Format |
Lyophilized |
Applikationen |
WB, FC, IHC, ELISA |
Specific against |
Human (Homo sapiens) |
Host |
Rabbit |
Isotype |
IgG |
Citations |
1. Bras, M., Yuste, V. J., Roue, G., Barbier, S., Sancho, P., Virely, C., Rubio, M., Baudet, S., Esquerda, J. E., Merle-Beral, H., Sarfati, M., Susin, S. A. Drp1 mediates caspase-independent type III cell death in normal and leukemic cells. Molec. Cell. Biol. 27: 7073-7088, 2007. 2. Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., Lindberg, F. P., Vignery, A. CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. J. Biol. Chem. 275: 37984-37992, 2000. 3. Oldenborg, P.-A., Zheleznyak, A., Fang, Y.-F., Lagenaur, C. F., Gresham, H. D., Lindberg, F. P. Role of CD47 as a marker of self on red blood cells. Science 288: 2051-2054, 2000. |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; IAP; Protein MER6; CD47; MER6 |
Lieferbar |
|
Specificity |
No cross reactivity with other proteins. |
Manufacturer - Category |
Primary Antibodies |
Manufacturer - Isotype |
Rabbit IgG |
Storage Conditions |
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. |
Observed Molecular Weight |
50 kDa |
Calculated Molecular weight |
132494 MW |
Clonality |
Polyclonal |
Application Details |
Western blot, 0.1-0.5?g/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5?g/ml
Flow Cytometry (Fixed), 1-3?g/1x106
ELISA, 0.1-0.5?g/ml |
Manufacturer - Gene Name |
CD47 |
Gene Full Name |
CD47 molecule |
Manufacturer - Specificity |
No cross reactivity with other proteins. |
Background |
CD47, also known as IAP or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 gene belongs to the immunoglobulin superfamily. This gene is mapped to 3q13.12. CD47 gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
Immunogen |
E.coli-derived human CD47 recombinant protein (Position: Q19-E323). |
Contents |
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.05 mg NaN3. |
Purification |
Immunogen affinity purified. |
Reconstitution |
Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml. |
Concentration |
Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml. |
Manufacturer - Research Category |
Cancer, Cell Type Markers, Immunology, Tumor Immunology |
Protein Name |
Leptin receptor |
Protein Function |
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. |
Subcellular Localization |
Cell membrane. Multi-pass membrane protein. |
Description |
Boster Bio Anti-CD47 Antibody Picoband® catalog # A00360-2. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. |
Tissue Specificity |
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. |
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