Antibodies

Anti-CD47 Antibody

Hersteller Boster
Kategorie
Typ Antibody Polyclonal
Specific against other
Isotype IgG
Format Lyophilized
Applikationen WB, FC, IF, IHC, ICC, ELISA, IHC-P
Menge 100ug/vial
Host Rabbit
ArtNr BOS-A00360-2
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Reactivity
Reacts with: human, mouse, rat
Description short
Rabbit IgG polyclonal antibody for CD47 detection. Tested with WB, IHC-P, IHC-F, ICC/IF, FCM, Direct ELISA in Human; Mouse; Rat.
Specificity
No cross reactivity with other proteins.
Application details
Western blot|0.1-0.5μ g/ml
Immunohistochemistry(Paraffin-embedded Section)|0.5-1μ g/ml
Immunohistochemistry(Frozen Section)|0.5-1μ g/ml
Immunocytochemistry/Immunofluorescence|2μ g/ml
Flow Cytometry|1-3ug/1x106
Direct ELISA|0.1-0.5μ g/ml
Notes
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
E.coli-derived human CD47 recombinant protein (Position: Q19-E323).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.
Background
CD47, also known as IAP or MER6, is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 gene belongs to the immunoglobulin superfamily. This gene is mapped to 3q13.12. CD47 gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes.
Reference
1. Bras, M., Yuste, V. J., Roue, G., Barbier, S., Sancho, P., Virely, C., Rubio, M., Baudet, S., Esquerda, J. E., Merle-Beral, H., Sarfati, M., Susin, S. A. Drp1 mediates caspase-independent type III cell death in normal and leukemic cells. Molec. Cell. Biol. 27: 7073-7088, 2007.
2. Han, X., Sterling, H., Chen, Y., Saginario, C., Brown, E. J., Frazier, W. A., Lindberg, F. P., Vignery, A. CD47, a ligand for the macrophage fusion receptor, participates in macrophage multinucleation. J. Biol. Chem. 275: 37984-37992, 2000.
3. Oldenborg, P.-A., Zheleznyak, A., Fang, Y.-F., Lagenaur, C. F., Gresham, H. D., Lindberg, F. P. Role of CD47 as a marker of self on red blood cells. Science 288: 2051-2054, 2000.
Gene Name
CD47
Protein Name
Leukocyte surface antigen CD47
Gene Full Name
CD47 molecule
Uniprot ID
Q08722
Entrez GeneID
961
Menge: 100ug/vial
Lieferbar: In stock
Listenpreis: 280,01 €
Preis: 280,01 €
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