Antibodies

Anti-CACNA2D2 Antibody

Hersteller Boster
Kategorie
Typ Antibody Primary
Specific against other
Isotype IgG
Format Lyophilized
Applikationen WB, IHC, ICC, ELISA
Menge 100ug/vial
Host Rabbit
ArtNr BOS-A01560-1
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Clonality
Polyclonal
Description short
Rabbit IgG polyclonal antibody for CACNA2D2 detection. Tested with WB, IHC-F, ICC, FCM, Direct ELISA in Human, Mouse, Rat.
Specificity
No cross reactivity with other proteins.
Reactivity
Reacts with: human, mouse, rat
Application details
Western blot|0.1-0.5μ g/ml
Immunohistochemistry(Frozen Section)|0.5-1μ g/ml
Immunocytochemistry|0.5-1μ g/ml
Flow Cytometry|1-3ug/1x106 cells
Direct ELISA|0.1-0.5μ g/ml
Notes
Tested Species: In-house tested species with positive results.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunogen
E. coli-derived human CACNA2D2 recombinant protein (Position: Q66-Q284).
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Purification
Immunogen affinity purified.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage
At -20C for one year. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for a longer time. Avoid repeated freezing and thawing.
Relevant detection systems
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(F) and ICC.
Background
Voltage-dependent calcium channel subunit alpha2delta-2 is a protein that in humans is encoded by the CACNA2D2 gene. This gene is mapped to 3p21.31. It encodes a member of the alpha-2/delta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. Research on a highly similar protein in rabbit suggests the protein described in this record is cleaved into alpha-2 and delta subunits. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.
Reference
1. Brill, J., Klocke, R., Paul, D., Boison, D., Gouder, N., Klugbauer, N., Hofmann, F., Becker, C.-M., Becker, K.Entla, a novel epileptic and ataxic Cacna2d2 mutant of the mouse. J. Biol. Chem. 279: 7322-7330, 2004.
2. Brodbeck, J., Davies, A., Courtney, J.-M., Meir, A., Balaguero, N., Canti, C., Moss, F. J., Page, K. M., Pratt, W. S., Hunt, S. P., Barclay, J., Rees, M., Dolphin, A. C. The ducky mutation in Cacna2d2 results in altered Purkinje cell morphology and is associated with the expression of a truncated alpha-2/delta-2 protein with abnormal function. J. Biol. Chem. 277: 7684-7693, 2002.
Gene Name
CACNA2D2
Protein Name
Voltage-dependent calcium channel subunit alpha-2/delta-2
Gene Full Name
calcium voltage-gated channel auxiliary subunit alpha2delta 2
Synonyms
Voltage-dependent calcium channel subunit alpha-2/delta-2; Voltage-gated calcium channel subunit alpha-2/delta-2; Voltage-dependent calcium channel subunit alpha-2-2; Voltage-dependent calcium channel subunit delta-2; CACNA2D2; KIAA0558
Uniprot ID
Q9NY47
Entrez GeneID
9254
Menge: 100ug/vial
Lieferbar: In stock
Listenpreis: 280,01 €
Preis: 280,01 €
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