Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody

Primary Antibodies

117849 MW

This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.

UBA1

Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Restore with deionized water (or equivalent) for reconstitution volume of 500 µL

Cell Biology, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers

Cytoplasm. Mitochondrion. Nucleus.

Boster Bio Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody (Catalog # A02810). Tested in IHC, WB applications. This antibody reacts with Human.