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Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody

Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody

Item no.	BOS-A02810
Manufacturer	Boster
Amount	500 ug
Category	Antibodies Cell Biology Cell Status Immunohistochemistry Western Blot Autophagy Primary Antibodies
Type	Antibody
Format	Lyophilized
Applications	WB, IHC
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Ubiquitin Activating Enzyme E1, A1S9, A1S9 protein, A1S9T and BN75 temperature sensitivity complementing, A1S9T, A1ST, GXP 1, UBE1
Available
Manufacturer - Category	Primary Antibodies
Storage Conditions	Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Observed Molecular Weight	117849 MW
Clonality	Polyclonal
Application notes	This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.
Application Details	ELISA: 1:2, 000 - 1:10, 000 IHC: 2 mg/ml - 20 µg/ml WB: 1:1, 000 - 1:5, 000
Manufacturer - Gene Name	UBA1
Gene Full Name	Ubiquitin-like modifier-activating enzyme 1
Background	Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation. E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2. Both variants encode the same protein.
Immunogen	Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
Contents	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Purification	This antibody is directed against human Ubiquitin Activating Enzyme E1 protein. The product was protein A purified from monospecific antiserum followed by further purification to remove the GST tag. A BLAST analysis was used to suggest that this antibody would react with Ubiquitin Activating Enzyme E1 protein from human (100%) rabbit (96%), mouse (95%), rat (95%) and dog (93%) based on a high degree of sequence homology. Cross-reactivity against this protein from other sources has not been determined.
Reconstitution	Restore with deionized water (or equivalent) for reconstitution volume of 500 µL
Concentration	1.2 mg/mL by UV absorbance at 280 nm
Manufacturer - Research Category	Cell Biology, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers
Protein Function	Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).
Subcellular Localization	Cytoplasm. Mitochondrion. Nucleus.
Short Description	Boster Bio Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody (Catalog # A02810). Tested in IHC, WB applications. This antibody reacts with Human.
Description	Boster Bio Anti-Ubiquitin Activating Enzyme E1 UBA1 Antibody (Catalog # A02810). Tested in IHC, WB applications. This antibody reacts with Human.
Tissue Specificity	Detected in erythrocytes (at protein level). Ubiquitous.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A02810-datasheet.pdf

BOS-A02810-safety-datasheet.pdf