Anti-Smac/Diablo Antibody

Primary Antibodies

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Liquid (sterile filtered)

This antibody was tested by immunoblot and reacts with human Smac/DIABLO protein. Lysates from human HeLa and LNCaP cells are positive for Smac/DIABLO. Other animal tissues have not been tested.

DIABLO

Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multi-cellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9.  Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

85 mg/mL by Refractometry

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity).

Boster Bio Anti-Smac/Diablo Antibody (Catalog # A03790). Tested in WB applications. This antibody reacts with Human.

Highest expression found in heart, liver, kidney and testis.