Anti-PBK1 RSL1D1 Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Polyclonal

ELISA: 1:2, 500 - 1:10, 000
IF Microscopy: 1:100
IP: 1:100
WB: 1:500 - 1:2, 000

PBK1 protein (also known as Ribosomal L1 domain-containing protein 1, cellular senescence-inhibited gene protein, and CATX-11) was isolated from highly invasive first trimester trophoblast cells and has been proposed to regulate their naturally occurring invasive behavior (Huch et al., 1998). PBK1 was also found to be over-expressed in non-small-cell lung cancer (NSCLC) cells (Petroziello et al., 2004). A recent study suggests that PBK1 may up-regulate the urokinase-type plasminogen activator (uPA) gene, which plays an important role in cellular matrix degradation and activation of other protease systems involved in cell invasion (Tong et al., 2005). The cellular localization dynamics of PBK1, as well as the data from its yeast homologue (Cic1p/Nsa3p) suggest that PBK1 is essential for ribosome biogenesis (Fatica et al., 2003). Thus, PBK1 could be controlling cell proliferation by regulating the level of ribosome production. It is hypothesized that PBK1 is involved in regulating both, cell proliferation and invasiveness, therefore playing a dual function in cancer cells transformation.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

1.1 mg/mL by UV absorbance at 280 nm

Boster Bio Anti-PBK1 RSL1D1 Antibody (Catalog # A07282). Tested in ELISA, IF, WB applications. This antibody reacts with Human.