Hersteller |
Boster
|
Kategorie |
|
Typ |
Antibody Monoclonal |
Specific against |
Human, Mouse, Rat |
Clon |
DCS-46 |
Isotype |
IgG1 |
Format |
Lyophilized |
Applikationen |
WB, ICC |
Menge |
100 ug/vial |
Host |
Mouse |
ArtNr |
BOS-MA1089 |
Targets |
SMAD4 |
eClass 6.1 |
32160702 |
eClass 9.0 |
42030590 |
Lieferbar |
|
Manufacturers Product Category |
Primary Antibodies, Mouse Monoclonal Antibodies |
Manufacturers Research Category |
Apoptosis, Cancer, Cancer Metabolism, Cardiogenesis, Cardiovascular, Cytoplasmic, Epigenetics and Nuclear Signaling, Hypertrophy, Metabolism, Metabolism Processes, Nuclear Signaling, Nuclear Signaling Pathways, Oncoproteins/Suppressors, Pathways and Processes, Response To Hypoxia, Signal Transduction, Signaling Pathway, Stem Cells, TGF Beta, Transcription Factors, Transcription Factors/Regulators, Tumor Suppressors, Vasculature |
Short Description |
Boster Bio Anti-Smad4 (DPC4) Antibody (Monoclonal, DCS-46) catalog # MA1089. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Description |
Boster Bio Anti-Smad4 (DPC4) Antibody (Monoclonal, DCS-46) catalog # MA1089. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat. |
Background |
SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Mutational inactivation of SMAD4 causes TGF-beta unresponsiveness and gave a basis for understanding the physiologic role of this gene in tumorigenesis. Mutations in DPC4 (SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases. |
Gene Name |
SMAD4 |
Application Details |
Immunocytochemistry , 1μg/ml, Human, - Western blot, 0.5-1μg/ml, Human, Mouse, Rat |
Application Notes |
Other applications have not been tested. Optimal dilutions should be determined by end users. |
Predicted Reactivity |
Bovine, Monkey |
Clonality |
Monoclonal |
Concentration |
Adding 1 ml of PBS buffer will yield a concentration of 100 μg/ml. |
Contents |
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
Immunogen |
Recombinant human Smad4 (DPC4). |
Purification |
Ascites |
Cross-reactivity |
No cross-reactivity with other proteins |
Storage |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Reconstitution |
Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
Gene Full Name |
Mothers against decapentaplegic homolog 4 |
Molecular Weight |
60469 MW |
Protein Function |
Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC- 3') within BMP response element (BMPRE) of cardiac activating regions. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8 (By similarity).. |
Subcellular Localization |
Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with R-SMAD. PDPK1 prevents its nuclear translocation.. |
Protein Name |
Mothers against decapentaplegic homolog 4 |
Sequence Similarities |
Belongs to the dwarfin/SMAD family. |
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