Anti-Phospho-DARPP 32 (Thr34) PPP1R1B Antibody

Primary Antibodies

22963 MW

Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr34. Immunolabeling is completely eliminated by treatment with ?-phosphatase.

PPP1R1B

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr-34. In contrast, when DARPP-32 is phosphorylated on Thr-75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Adapters, Cytoplasmic, Metabolism, Neuroscience, Neurotransmission, Obesity, Receptors / Channels, Signal Transduction

Inhibitor of protein-phosphatase 1.

Boster Bio Anti-Phospho-DARPP 32 (Thr34) PPP1R1B Antibody (Catalog # P03424-1). Tested in WB applications. This antibody reacts with Mouse, Rat.

Bovine, Canine, Chicken, Human, Primate