Anti-Phospho-GABAB R2 (Ser783) GABBR2 Antibody

Primary Antibodies

105821 MW

Specific for endogenous levels of the ~102 kDa GABAB R2 phosphorylated at Ser783. Immunolabeling is completely eliminated by treatment with ?-phosphatase.

GABBR2

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-B receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABA-B receptors and phosphorylates Ser-783 in the cytoplasmic tail of the R2 subunit and that Ser-783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of Ser-783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007).

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Amino Acids, Ligand-Gated Ion Channels, More Gpcr, Neuroscience, Neurotransmission, Neurotransmitter, Receptors / Channels

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception.

Boster Bio Anti-Phospho-GABAB R2 (Ser783) GABBR2 Antibody (Catalog # P03830). Tested in WB, IHC, ICC applications. This antibody reacts with Mouse, Rat.

Highly expressed in brain, especially in cerebral cortex, thalamus, hippocampus, frontal, occipital and temporal lobe, occipital pole and cerebellum, followed by corpus callosum, caudate nucleus, spinal cord, amygdala and medulla. Weakly expressed in heart, testis and skeletal muscle.