Anti-PP2A-alpha/PPP2CA Antibody Picoband®

ArtNr	BOS-PA1068
Hersteller	Boster
Menge	100 ug/vial
Kategorie	Oncology Food Safety Allergenes Neuroscience Protein Aggregation, Clearance & Misfolding Antibodies By Cell Type Carcinoma Hematology Lymphoma Sarcoma Immunohistochemistry Western Blot Primary Antibodies By Organ Lung Cancer
Typ	Antibody Polyclonal
Format	Lyophilized
Applikationen	WB, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Sensitivity	<1pg/ml
Citations	1. Stone SR, Mayer R, Wernet W, Maurer F, Hofsteenge J, Hemmings BA. The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A alpha catalytic subunit. Nucleic Acids Res. 1988 Dec 9; 16(23):11365. 2. Jones TA, Barker HM, da Cruz e Silva EF, Mayer-Jaekel RE, Hemmings BA, Spurr NK, Sheer D, Cohen PT. Localization of the genes encoding the catalytic subunits of protein phosphatase 2A to human chromosome bands 5q23-->q31 and 8p12-->p11.2, respectively. Cytogenet Cell Genet. 1993; 63(1):35-41. 3. Khew-Goodall Y, Mayer RE, Maurer F, Stone SR, Hemmings BA. Structure and transcriptional regulation of protein phosphatase 2A catalytic subunit genes. Biochemistry. 1991 Jan 8; 30(1):89-97. 4. Arino J, Woon CW, Brautigan DL, Miller TB Jr, Johnson GL. Human liver phosphatase 2A: cDNA and amino acid sequence of two catalytic subunit isotypes. Proc Natl Acad Sci U S A. 1988 Jun; 85(12):4252-6. 5. Bryant JC, Westphal RS, Wadzinski BE. Methylated C-terminal leucine residue of PP2A catalytic subunit is important for binding of regulatory Balpha subunit. Biochem J. 1999 Apr 15; 339 ( Pt 2):241-6. 6. Gergs U, Boknik P, Buchwalow I, Fabritz L, Matus M, Justus I, Hanske G, Schmitz W, Neumann J. Overexpression of the catalytic subunit of protein phosphatase 2A impairs cardiac function. J Biol Chem. 2004 Sep 24; 279(39):40827-34.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform;PP2A-alpha;3.1.3.16;Replication protein C;RP-C;PPP2CA;
Similar products	catalytic subunit, Protein phosphatase 2, PP2A alpha antibody, PP2A-alpha antibody, PP2AA_HUMAN antibody, PP2Aalpha antibody, PP2Ac antibody, PP2CA antibody, PP2Calpha antibody, PPP2CA antibody, Protein phosphatase 2(formerly 2A) catalytic subunit alpha isoform antibody, Protein phosphatase 2 catalytic subunit alpha isoform antibody, alpha isozyme antibody, Protein phosphatase 2A catalytic subunit, alpha isoform antibody, Replication protein C antibody, RP C antibody, RP-C antibody, RPC antibody, Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
Lieferbar
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	36 kDa
Calculated Molecular weight	35594 MW
Clonality	Polyclonal
Application Details	Western blot, 0.1-0.5?g/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1?g/ml, Human, Rat, By Heat
Manufacturer - Gene Name	PPP2CA
Gene Full Name	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Manufacturer - Specificity	No cross reactivity with other proteins.
Background	The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
Immunogen	A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A-alpha, identical to the related rat and mouse sequences.
Contents	Each vial contains 5 mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg NaN3.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Cancer, Cardiovascular, Cell Biology, Cell Cycle, Contractility, Cytoplasmic, Epigenetics and Nuclear Signaling, Intracellular Signaling, Kinases/Phosphatases, Neuroscience, Neurotransmission, Protein Phosphorylation, Ser/Thr Phosphatases, Signal Transduction, Signaling Pathways, Stem Cells, TGF Beta
Protein Name	Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PP2A-alpha)
Protein Function	PP2A is the major phosphatase for microtubule-associated proteins (MAPs). PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Activates RAF1 by dephosphorylating it at 'Ser-259'.
Subcellular Localization	Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity).
Description	Boster Bio Anti-PP2A-alpha/PPP2CA Antibody catalog # PA1068. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Tissue Specificity	Heart, liver, skeletal muscle, kidney, and pancreas. Lower level in brain, placenta and lung.

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