Human Cathepsin D PicoKine ELISA Kit

Hersteller Boster
Kategorie Produkte / ELISA
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96 Wells
ArtNr BOS-EK0672
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Gene name
CTSD
Assay range
156pg/ml-10, 000pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of human Cathepsin D in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Uniprot
P07339
Immunogen
Expression system for standard: NSO; Immunogen sequence: L21-L412
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<10pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Anticoagulant
heparin or EDTA
Assay procedure step1
10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml
Background
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Research category
|neuroscience|cell adhesion proteins|membrane proteins| signal transduction|cytoskeleton / ecm|extracellular matrix|ecm enzymes|other enzymes| cancer|invasion/microenvironment| cell biology|proteolysis / ubiquitin|proteolytic enzymes|cysteine protease|cathepsins
Synonyms
Cathepsin D; 3.4.23.5; Cathepsin D light chain; Cathepsin D heavy chain; CTSD; CPSD;
Gene full name
Cathepsin D
MW
44552 MW
Protein function
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Subcellular localization
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). .
Tissue specificity
Expressed in the aorta extrcellular space (at protein level). .
Protein name
Cathepsin D
Sequence similarities
Belongs to the peptidase A1 family.
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(ng/ml)|1.24|3.37|6.73|1.38|3.51|6.82
Standard deviation|0.035|0.108|0.236|0.047|0.130|0.28
CV(%)|2.8|3.2|3.5|3.4|3.7|4.1
Sample data
Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000
O.D.|0.000|0.075|0.173|0.287|0.525|0.941|1.542|2.314
Tmb Incubation time
25-30min
Principle
monoclonal antibody from mouse|polyclonal antibody from goat
Normal range citations
11331669
Menge: 96 Wells
Lieferbar: In stock
Listenpreis: 390,91 €
Rabatt: -10%
Preis: 351,82 €
lieferbar

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Sicherheitsdatenblatt

BOS-EK0672.pdf

Arbeitsanleitung

BOS-EK0672.pdf