Human TNFSF12 PicoKine ELISA Kit

Hersteller Boster
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 96 Wells
ArtNr BOS-EK0920
Eclass 6.1 32160605
Eclass 9.0 32160605
Lieferbar
Gene name
TNFSF12
Assay range
62.5pg/ml-4000pg/ml
Components needed
  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
    • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μ l of purified acetic acid or 700μ l of concentrated hydrochloric acid to 1000ml H2
    • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
Conjugate
No
Description
For quantitative detection of human TNFSF12 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Uniprot
O43508
Immunogen
Expression system for standard: E.coli; Immunogen sequence: S94-H249
Storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactivity
There is no detectable cross-reactivity with other relevant proteins.
Sensitivity
<10pg/ml
Kit components
10ng/tube
Sample type
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Anticoagulant
heparin or EDTA
Assay procedure step1
4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml
Background
Tumor necrosis factor ligand superfamily member 12 also known as TNF-related weak inducer of apoptosis(TWEAK) is a protein that in humans is encoded by the TNFSF12 gene. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator ofangiogenesis. The TNFSF12 gene lies 878 bp upstream of the putative transcriptional start site of the TNFSF13 gene on chromosome 17p13.1.
Research category
|cell biology|apoptosis|extracellular signals|death ligands| cancer|growth factors|tnf|invasion/microenvironment|cell death
Synonyms
Tumor necrosis factor ligand superfamily member 12; APO3 ligand; TNF-related weak inducer of apoptosis; TWEAK; Tumor necrosis factor ligand superfamily member 12, membrane form; Tumor necrosis factor ligand superfamily member 12, secreted form; TNFSF12; APO3L, DR3LG; UNQ181/PRO207;
Gene full name
Tumor necrosis factor ligand superfamily member 12
MW
27216 MW
Protein function
Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion. .
Subcellular localization
Cell membrane ; Single-pass type II membrane protein .
Tissue specificity
Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
Protein name
Tumor necrosis factor ligand superfamily member 12
Sequence similarities
Belongs to the tumor necrosis factor family.
Intra inter assay cv
|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|588|1202|2235|634|1127|2446
Standard deviation|26.46|65|84.93|37.41|72.13|115
CV(%)|4.5|5.4|3.8|5.9|6.4|4.7
Sample data
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000
O.D.|0.028|0.085|0.129|0.209|0.380|0.855|1.427|2.319
Tmb Incubation time
20-25min
Principle
monoclonal antibody from mouse|polyclonal antibody from goat
Normal range citations
23800379

Sicherheitsdatenblatt

BOS-EK0920.pdf

Arbeitsanleitung

BOS-EK0920.pdf

Menge: 96 Wells
Lieferbar: In stock
Listenpreis: 369,00 €
Preis: 369,00 €
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