Human PLA2G2A ELISA Kit PicoKine®

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

phospholipase A2 group IIA

Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 1p36.13. The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5'UTRs have been found for this gene.

Cancer, Cardiovascular, Lipases, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Lipids/Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Signal Transduction, Signaling Pathway

Secreted, Cell membrane, Peripheral membrane protein

cell culture supernatants, serum and plasma (heparin, EDTA).

Intra-Assay Precision | Inter-Assay Precision
Sample | 1 | 2 | 3 | 1 | 2 | 3
n | 16 | 16 | 16 | 24 | 24 | 24
Mean (pg/ml) | 249 | 1511 | 3890 | 265 | 1585 | 4121
Standard deviation | 13.20 | 93.68 | 171.16 | 18.82 | 76.08 | 259.62
CV (%) | 5.3% | 6.2% | 4.4% | 7.1% | 4.8% | 6.3%

A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat

10 ng/tube

Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer (optional).
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.