HER2 (Phospho-Tyr1248) Colorimetric Cell-Based ELISA Kit

Store at 4°C for up to 6 months.

ERBB2

Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

The HER2 (Phospho-Tyr1248) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor HER2 (Phospho-Tyr1248) protein expression profile in cells. The kit can be used for measuring the relative amounts of HER2 (Phospho-Tyr1248) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on HER2 (Phospho-Tyr1248).

Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.