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Human M-CSF ELISA Kit PicoKine®

Human M-CSF ELISA Kit PicoKine®

ArtNr	BOS-FEK0444
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Quantity options	96 wells/kit, with removable strips. 96 wells/kit, with removable strips.
Kategorie	Oncology Food Safety Hormones ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Lung Cancer Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<10 pg/ml
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Macrophage colony-stimulating factor 1,CSF-1,M-CSF,MCSF,Lanimostim,Processed macrophage colony-stimulating factor 1,CSF1
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	60179 MW
Gene Name	CSF1
Gene Full Name	Macrophage colony-stimulating factor 1
Manufacturer - Specificity	Natural and recombinant human M-CSF
Background	M-CSF, also called CSF1, has a role in development of the placenta. Uterine CSF1 concentration is regulated by a synergistic action of estradiol and progesterone. CSF1 is produced by uterine glandular epithelial cells. It had been found that FMS, the CSF1 receptor, is expressed in placenta and choriocarcinoma cell lines1. The CSF1 gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb2. And there are 2 forms of CSF1, with 224 and 522 amino acids, resulting from alternative splicing3.
Immunogen	Expression system for standard: E.coli; Immunogen sequence: E33-S190
Protein Name	Macrophage colony-stimulating factor 1
Protein Function	Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
Subcellular Localization	Cell membrane; Single-pass type I membrane protein.
Description	Human M-CSF ELISA Kit PicoKine® (120 minutes, 96 Tests). Quantitate Human CSF1 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Sample Data	Concentration (pg/ml) \| 0 \| 62.5 \| 125 \| 250 \| 500 \| 1000 \| 2000 \| 4000 O.D. \| 0.004 \| 0.128 \| 0.299 \| 0.537 \| 0.992 \| 1.347 \| 1.803 \| 2.095
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 152 \| 681 \| 1139 \| 140 \| 716 \| 1136 Standard deviation \| 8.05 \| 32.68 \| 52.39 \| 9.1 \| 40.09 \| 52.25 CV (%) \| 5.3% \| 4.8% \| 4.6% \| 6.5% \| 5.6% \| 4.6%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 152 \| 153 \| 143 \| 153 \| 150 \| 4.2 \| 2.8% Sample 2 \| 681 \| 697 \| 651 \| 751 \| 695 \| 36.3 \| 5.2% Sample 3 \| 1139 \| 1296 \| 1284 \| 1344 \| 1265 \| 76.54 \| 6% *number of samples for each test n=16.
Capture antibody and Detection antibody	monoclonal antibody from mouse\|polyclonal antibody from goat
Kit Components	Description\|Quantity 96-well plate\|1 lyophilized Standard\| Biotinylated detection antibody (50x)\|100 ul Avidin-Biotin-Peroxidase Complex (30x)\|400 µl Sample diluent\|30 ml Antibody diluent\|12 ml Avidin-Biotin-Peroxidase Diluent\|12 ml Wash Buffer (25x)\|20 ml Color Developing Reagent (TMB)\|10 ml Stop Solution\|10 ml Plate Sealers\|4
Standard	10 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-FEK0444-datasheet.pdf

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