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Human CD21/CR2 PicoKine® Quick ELISA Kit

Human CD21/CR2 PicoKine® Quick ELISA Kit

ArtNr	BOS-FEK1315
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Kategorie	Neuroscience Neuroinflammation ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	<20 pg/ml
Sequence	Belongs to the receptors of complement activation (RCA) family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Complement receptor type 2,Cr2,Complement C3d receptor,Epstein-Barr virus receptor,EBV receptor,CD21,CR2,C3DR
Lieferbar
Manufacturer - Category	Quick ELISA Kits
Storage Conditions	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Calculated Molecular weight	112916 MW
Gene Name	CR2
Gene Full Name	Complement receptor type 2
Manufacturer - Specificity	Natural and recombinant human CD21
Background	Complement receptor type 2 (CR2), also known as C3DR or CD21, is a protein that in humans is encoded by the CR2 gene. It is mapped to 1q32.2. CR2 is involved in the complement system. It binds to iC3b (inactive derivative of C3b), C3dg, or C3d. CR2 is the membrane protein on B lymphocytes to which the Epstein-Barr virus (EBV) binds during infection of these cells. CR2, together with CD19, CD81, and CD225, forms the B-cell coreceptor complex, which lowers the activation threshold of the B-cell antigen receptor. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9).
Immunogen	Expression system for standard: CHO; Immunogen sequence: Ile21-71
Protein Name	Complement receptor type 2
Protein Function	Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.
Subcellular Localization	Membrane; Single-pass type I membrane protein.
Description	Human CD21/CR2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Tissue Specificity	Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.
Sample Type	cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Sequence Similarities	Belongs to the receptors of complement activation (RCA) family.
Sample Data	Concentration (pg/ml) \| 0 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1, 000 \| 2, 000 O.D. \| 0.014 \| 0.096 \| 0.168 \| 0.317 \| 0.565 \| 0.938 \| 1.757 \| 2.382
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean(pg/ml) \| 59 \| 260 \| 913 \| 58 \| 268 \| 861 Standard deviation \| 3.36 \| 12.22 \| 49.30 \| 4.40 \| 13.93 \| 55.96 CV(%) \| 5.7% \| 4.7% \| 5.4% \| 7.6% \| 5.2% \| 6.5%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 1242 \| 1468 \| 1273 \| 1362 \| 1336 \| 87.89 \| 6.5% Sample 2 \| 7916 \| 7621 \| 6548 \| 6832 \| 7229 \| 558.34 \| 7.7% Sample 3 \| 24012 \| 23247 \| 26467 \| 22418 \| 24036 \| 1512.51 \| 6.2% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity\|Volume Anti-tag Pre-coated 96-well Strip Microplate\|1\|12 strips of 8 wells Standard\|\|2 Antibody A\|1\|3ml Antibody B\|1\|3ml Sample Diluent\|1\|15ml TBS-T Wash Buffer (25x)\|1\|12ml Color Developing Reagent (TMB)\|1\|10ml Stop Solution\|1\|10ml Adhesive Plate Sealers\|2\|Piece
Standard	50 ng/tube
Anticoagulant	heparin or EDTA
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with other relevant proteins.

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BOS-FEK1315-datasheet.pdf

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