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Rat NGF/NGF Beta ELISA Kit PicoKine®

Rat NGF/NGF Beta ELISA Kit PicoKine®

ArtNr	BOS-EK0471
Hersteller	Boster
Menge	96 wells/kit, with removable strips.
Quantity options	96 wells/kit, with removable strips. 5 plates/kit
Kategorie	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer Immunostaining Neuronal Cell Markers
Typ	Elisa-Kit
Applikationen	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	<1 pg/ml
Sequence	Belongs to the NGF-beta family.
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Beta-nerve growth factor,Beta-NGF,Ngf,Ngfb
Lieferbar
Manufacturer - Category	PicoKine® ELISA Kits
Storage Conditions	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Calculated Molecular weight	27010 MW
Gene Name	NGF
Gene Full Name	Beta-nerve growth factor
Manufacturer - Specificity	Natural and recombinant rat NGF
Background	Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130, 000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
Immunogen	Expression system for standard: NS0; Immunogen sequence: S122-G241
Manufacturer - Research Category	Growth and Development, Mesenchymal Stem Cells, Neural Stem Cells, Neurology Process, Neuroscience, Stem Cells, Surface Molecules
Protein Name	Beta-nerve growth factor
Protein Function	Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI.
Subcellular Localization	Secreted.
Description	Rat NGF/NGF Beta ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ngf in cell culture supernatants and serum. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Sample Type	cell culture supernatants and serum.
Sequence Similarities	Belongs to the NGF-beta family.
Sample Data	Concentration (pg/ml) \| 0 \| 15.6 \| 31.2 \| 62.5 \| 125 \| 250 \| 500 \| 1000 O.D. \| 0.079 \| 0.133 \| 0.190 \| 0.296 \| 0.521 \| 0.876 \| 1.414 \| 2.025
Intra Assay Consistency & Inter Assay Consistency	Intra-Assay Precision \| Inter-Assay Precision Sample \| 1 \| 2 \| 3 \| 1 \| 2 \| 3 n \| 16 \| 16 \| 16 \| 24 \| 24 \| 24 Mean (pg/ml) \| 33 \| 191 \| 480 \| 29 \| 201 \| 491 Standard deviation \| 1.84 \| 8.02 \| 32.64 \| 1.62 \| 11.65 \| 35.84 CV (%) \| 4.2% \| 4.2% \| 6.8% \| 5.6% \| 5.8% \| 7.3%
Reproducibility	Lots \| Lot 1 (pg/ml) \| Lot 2 (pg/ml) \| Lot 3 (pg/ml) \| Lot 4 (pg/ml) \| Mean (pg/ml) \| Standard Deviation \| CV (%) Sample 1 \| 33 \| 32 \| 33 \| 35 \| 33 \| 1.08 \| 3.2% Sample 2 \| 191 \| 172 \| 185 \| 192 \| 185 \| 7.96 \| 4.3% Sample 3 \| 480 \| 451 \| 440 \| 436 \| 451 \| 17.21 \| 3.8% *number of samples for each test n=16.
Capture antibody and Detection antibody	polyclonal antibody from goat\|polyclonal antibody from goat
Kit Components	Description\|Quantity Pre-coated 96-well strip microplate\|1 Standard\|2 vials Biotinylated antibody (100x)\|100ul Avidin-Biotin-Peroxidase Complex (100x)\|100ul Sample Diluent\|30ml Antibody Diluent\|12ml Avidin-Biotin-Peroxidase Diluent\|12ml Color Developing Reagent (TMB)\|10ml Stop Solution\|10ml Wash Buffer (25x)\|20ml Adhesive plate sealers\|4
Standard	10 ng/tube
Material Required But Not Provided	Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.
Manufacturer - Cross Reactivity	There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BOS-EK0471.pdf

BOS-EK0471-datasheet.pdf

BOS-EK0471-safety-datasheet.pdf