Vergleich

Endothelium

ArtNr USB-E2292-02A
Hersteller United States Biological
Menge 1 ml
Kategorie
Typ Antibody Monoclonal
Format Liquid
Applikationen FC, IHC, IC
Clon EN4
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
Purity Purified by Protein G affinity chromatography.
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Versandbedingung Gekühlt
Lieferbar
Manufacturer - Type
Mab
Manufacturer - Category
Antibodies / Antibodies-Cell Markers
Shipping Temperature
Blue Ice
Storage Conditions
-20°C
Grade
Affinity Purified
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
EU Commodity Code
30021010
Immunogen
Human Endothelium
Specificity
Recognizes human Endothelium. Stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly mouse fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130kD CD31 antigen. Also binds to guinea-pig and feline endothelium, but not to rabbit, bovine, sheep, canine, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
Description
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:20 works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 ml
Lieferbar: In stock
lieferbar

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