Comparison

Endothelium

Item no. USB-E2292-02A
Manufacturer United States Biological
Amount 1 ml
Category
Type Antibody Monoclonal
Format Liquid
Applications FC, IHC, IC
Clone EN4
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1
Purity Purified by Protein G affinity chromatography.
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Shipping Condition Cool pack
Available
Manufacturer - Type
Mab
Manufacturer - Category
Antibodies / Antibodies-Cell Markers
Shipping Temperature
Blue Ice
Storage Conditions
-20°C
Grade
Affinity Purified
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
EU Commodity Code
30021010
Immunogen
Human Endothelium
Specificity
Recognizes human Endothelium. Stains all human blood vessels including brain microvessels. Both large and small vessels are equally reactive. The EN4 antigen is preserved in endothelial cells in culture unlike the PAL-E antigen which is lost. It stains strongly mouse fibroblasts transfected with the human CD31 gene. On surface-iodinated Jurkat T cells it recognizes the 130kD CD31 antigen. Also binds to guinea-pig and feline endothelium, but not to rabbit, bovine, sheep, canine, rat or mice endothelial cells. No other structures in the skin, heart, kidney, tonsils or spleen are stained with this antibody.
Description
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:20 works well on acetone fixed or unfixed cryostat sections, and periodate/lysine/paraformaldehyde-fixed sections, in which membrane antigens and the structures of vessels and adjacent tissues are optimally preserved.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 ml
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close