Anti-Human IFN-alpha/beta R1 Clone MMHAR-1 mAb

Western Blot: 1-2 ig/ml with the appropriate secondary reagents to detect human IFN-a/a R1.Flow Cytometry: Dilute antibody to 25 ig/ml and add 10il of this solution to 1 - 2.5 x 105 cells in a total reaction volume not exceeding 200il. The binding of the unlabeled antibody may be visualized by adding 10il of a 25 ig/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to fluorochrome.Direct ELISA: 0.5-1.0 ig/ml with the appropriate secondary reagents to detect human IFN-a/a R1.Optimal dilutions should be determined by each laboratory for each application

Lyophilized from a 0.2um filtered solution in phosphate-buffered saline (PBS) containing 5% trehalose

Protein G affinity chromatography

0.5 mg/ml, after reconstitution with 1ml sterile PBS