Antibodies

Anti-Human IFN-alpha/beta R1 Clone MMHAR-1 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Clon MMHAR-1
Isotype IgG1
Applikationen WB, FC, ELISA
Menge 0.5 mg
Host Mouse
ArtNr CS-CMI224
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
WB, FC, ELISA
Description
Mouse Anti-Human Interferon Alpha/Beta R1
Formulation
Lyophilized from a 0.2um filtered solution in phosphate-buffered saline (PBS) containing 5% trehalose
Specificity
Detects human interferon alpha/beta R1, in direct ELISAs, this antibody shows no cross reactivity with Hu-IFN-gamma R1, Hu-IFN-gamma R2, Mu-IFN-alpha/beta R2, Hu-IL-10 Rbeta, Hu-IL-10 Ralpha or Hu-IL-20 Rbeta.
Immunogen
Sf 21-derived Hu-IFN-alpha/beta R1 extracellular domain
Purification
Protein G affinity chromatography
Storage and Stability
After receipt, this product should be kept at -20C for retention of full activity. Upon reconstitution with sterile PBS, the antibody can be stored at 2-8C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquoted and frozen at -20C to -80C in a manual defrost freezer for 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Concentration
0.5 mg/ml, after reconstitution with 1ml sterile PBS
Application Notes
Western Blot: 1-2 ig/ml with the appropriate secondary reagents to detect human IFN-a/a R1.Flow Cytometry: Dilute antibody to 25 ig/ml and add 10il of this solution to 1 - 2.5 x 105 cells in a total reaction volume not exceeding 200il. The binding of the unlabeled antibody may be visualized by adding 10il of a 25 ig/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to fluorochrome.Direct ELISA: 0.5-1.0 ig/ml with the appropriate secondary reagents to detect human IFN-a/a R1.Optimal dilutions should be determined by each laboratory for each application
Menge: 0.5 mg
Lieferbar: In stock
Listenpreis: 3.213,06 €
Preis: 3.213,06 €
lieferbar

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