Comparison

Anti-Human IFN-alpha/beta R1 Clone MMHAR-1 mAb

Manufacturer Cell Sciences
Category
Type Antibody Monoclonal
Specific against Human
Clone MMHAR-1
Isotype IgG1
Applications WB, FC, ELISA
Amount 0.5 mg
Host Mouse
Item no. CS-CMI224
Targets IFNB1, CD1b
eClass 6.1 32160702
eClass 9.0 42030590
Available
Application Notes
Western Blot: 1-2 ig/ml with the appropriate secondary reagents to detect human IFN-a/a R1.Flow Cytometry: Dilute antibody to 25 ig/ml and add 10il of this solution to 1 - 2.5 x 105 cells in a total reaction volume not exceeding 200il. The binding of the unlabeled antibody may be visualized by adding 10il of a 25 ig/ml stock solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to fluorochrome.Direct ELISA: 0.5-1.0 ig/ml with the appropriate secondary reagents to detect human IFN-a/a R1.Optimal dilutions should be determined by each laboratory for each application
Concentration
0.5 mg/ml, after reconstitution with 1ml sterile PBS
Description
Mouse Anti-Human Interferon Alpha/Beta R1
Formulation
Lyophilized from a 0.2um filtered solution in phosphate-buffered saline (PBS) containing 5% trehalose
Immunogen
Sf 21-derived Hu-IFN-alpha/beta R1 extracellular domain
Purification
Protein G affinity chromatography
Storage and Stability
After receipt, this product should be kept at -20C for retention of full activity. Upon reconstitution with sterile PBS, the antibody can be stored at 2-8C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquoted and frozen at -20C to -80C in a manual defrost freezer for 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Specificity
Detects human interferon alpha/beta R1, in direct ELISAs, this antibody shows no cross reactivity with Hu-IFN-gamma R1, Hu-IFN-gamma R2, Mu-IFN-alpha/beta R2, Hu-IL-10 Rbeta, Hu-IL-10 Ralpha or Hu-IL-20 Rbeta.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.5 mg
Available: In stock
available

Delivery expected until 5/30/2024 

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