Antibodies

Mouse Anti-Human PARP Clone C-2-10 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Clon C-2-10
Isotype IgG1
Applikationen WB, ICC
Menge 100 ug
Host Mouse
ArtNr CS-CMP134
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot Immunocytochemistry
Description
Mouse Anti-Multispecies PARP Clone C-2-10 monoclonal antibody. PARP is a 116 kDa, highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+-dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in apoptosis responses of cells. This protein can be cleaved by many caspases, in vitro, and is one of the main cleavage targets of caspase 3, in vivo . The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis, in vitro .
Formulation
Liquid in PBS + 0.5% BSA + 0.05% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Recognizes the full length (116 kDa) and the apoptosis-related cleavage fragment (85 kDa) of PARP.
Immunogen
Purified calf thymus PARP
Purification
Protein A purified
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles .
Cross-Reactivity
Human, mouse, rat, monkey, and hamster
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 582,72 €
Preis: 582,72 €
lieferbar

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Arbeitsanleitung

CS-CMP134.pdf