Mouse Anti-Human STEAP1 Clone J2D2 mAb

Immunohistochemistry, Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) ImmunofluorescenceImmunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 antibody (J2D2) Western Blot Analysis Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Human, Rat

Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.

By protein-G affinity chromatography