Mouse Anti-Human STEAP1 Clone J2D2 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG2b kappa
Applikationen WB, IF, ELISA
Menge 100 ul
Host Mouse
ArtNr CS-CMS103B
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
ELISA, WB, Immunofluorescence, Immunohistochemistry
Description
Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.
Formulation
Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen
Recombinant human STEAP1 (1-80 aa) purified from, E. coli
Purification
By protein-G affinity chromatography
Storage and Stability
Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.
Concentration
1 mg/ml
Cross-Reactivity
Human, Rat
Application Notes
Immunohistochemistry, Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) ImmunofluorescenceImmunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 antibody (J2D2) Western Blot Analysis Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Menge: 100 ul
Lieferbar: In stock
Listenpreis: 543,94 €
Preis: 543,94 €
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