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Mouse Anti-Human STEAP1 Clone J2D2 mAb

Mouse Anti-Human STEAP1 Clone J2D2 mAb

Manufacturer	Cell Sciences
Category	Antibodies Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Monoclonal
Specific against	Human
Isotype	IgG2b Kappa
Applications	WB, ELISA
Amount	100 ul
Host	Mouse
Item no.	CS-CMS103B
eClass 6.1	32160702
eClass 9.0	42030590
Available
Application Notes	Immunohistochemistry, Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) ImmunofluorescenceImmunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 antibody (J2D2) Western Blot Analysis Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Concentration	1 mg/ml
Cross-Reactivity	Human, Rat
Description	Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.
Formulation	Liquid. Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. Precaution: Sodium azide is a poisonous and hazardous substance which should be handled by trained staff only.
Immunogen	Recombinant human STEAP1 (1-80 aa) purified from, E. coli
Purification	By protein-G affinity chromatography
Storage and Stability	Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ul

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Delivery expected until 5/23/2024