|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Antibody |
|
Specific against |
Human |
|
Clon |
3 |
|
Isotype |
IgG1 |
|
Menge |
50 ug |
|
Host |
Mouse |
|
ArtNr |
CS-CMV012BT |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Applications |
FAC, E |
| Description |
Monoclonals were produced with the help of BALB/c mice using recombinant human soluble extracellular KDR (110 kDa) as the immunizing antigen. Mouse IgG1 antibody (clone 3) from hybridomas was purified from cell culture supernatant by Protein G chromatography and then biotinylated using a standard protocol. |
| Formulation |
Lyophilized from a 0.2 um sterile filtered 0.1 M Tris-C1, 0.2 M NacI, 0.02% NaN3 buffer solution (pH.4), containing 50 ug BSA per 1 ug antibody. |
| Specificity |
Human |
| Immunogen |
Recombinant human soluble KDR protein. |
| Purification |
Biospecific affinity chromatography. |
| Reconstitution |
The biotinylated antibody should be reconstituted to a concentration of 50 ug/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20C. Avoid more than one freeze-thaw cycle . |
| Storage and Stability |
The lyophilized antibody is best stored desiccated below 0C. Reconstituted anti-VEGFR-2/KDR is stable at 4C for >one month or can be stored in working aliquots at 20C for more than six months. |
