Antibodies

Rabbit Anti-Human PARP Affinity Purified pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Human
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPP139
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot
Description
Rabbit Anti-Human PARP Affinity Purified polyclonal antibody. PARP is a 116 kDa, highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+-dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in apoptosis responses of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase 3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.
Formulation
Liquid in PBS + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP.
Immunogen
Synthetic peptide corresponding to caspase cleavage site in human PARP
Purification
Biospecific affinity chromatography
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 599,26 €
Preis: 599,26 €
lieferbar

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Arbeitsanleitung

CS-CPP139.pdf