Rabbit Anti-Human PARP Affinity Purified pAb

Rabbit Anti-Human PARP Affinity Purified polyclonal antibody. PARP is a 116 kDa, highly conserved nuclear enzyme present in higher eukaryotes. The enzyme is a Zn2+-dependent DNA binding protein that recognizes DNA strand breaks and is implicated in DNA repair and in apoptosis responses of cells. This protein can be cleaved by many caspases in vitro and is one of the main cleavage targets of caspase 3 in vivo. The cleavage occurs between ASP214 and Gly 215, which separates PARP’s N-terminal DNA binding domain (24 kDa) from its C-terminal catalytic domain (89 kDa). It has been shown that cleavage of PARP facilitates cellular disassembly and inhibition of PARP cleavage attenuates apoptosis in vitro.

Synthetic peptide corresponding to caspase cleavage site in human PARP

Recognizes the full length (116 kDa) and the cleaved fragment (85 kDa) of human PARP.