Rabbit Anti-Human FLIP Gamma delta (CT) Affinity Purified pAb

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)-containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and c-FLIPs, respectively1, 2. The human FLIPs were also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH, CLARP and usurpin3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and –10, and potently inhibits apoptosis induced by all known death receptors CD95, DR3, TRAIL-R and TNFR1. Four splice variants of c-FLIPs have been identified and termed FLIPa, b, g, and d, respectively8. This antibody can be used for detection of FLIP by Western blot at 1:1000 dilution. Jurkat cell lysate can be used as positive control and 35 kDa and 25 kDa bands can be detected. This antibody recognizes human and murine FLIPg and FLIPd but not FLIPa and FLIPb. For research use only.