|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Antibody |
|
Specific against |
Mouse |
|
Isotype |
IgG |
|
Applikationen |
WB |
|
Menge |
100 ug |
|
Host |
Rabbit |
|
ArtNr |
CS-CPC126 |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Applications |
Western Blot |
| Description |
Rabbit Anti-Mouse Caspase 9 polyclonal antibody. Caspase 9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase 9/APAF1. The complex processes procaspase 9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase 9 further cleaves other caspase members including caspase 3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase 9 can also be cleaved in vivo by caspase 3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway. |
| Formulation |
Liquid in PBS, pH 7.2 + 30% glycerol + 0.5% BSA + 0.01% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only. |
| Specificity |
Detects 45 kDa Procaspase 9, 35/37 kDa cleaved large fragment, and the 25 kDa fully cleaved Caspase 9. |
| Immunogen |
Synthetic peptide surrounding amino acid 345 of mouse Caspase 9 |
| Purification |
Protein A purified |
| Storage and Stability |
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles. |
| Cross-Reactivity |
Reacts with mouse and rat. |
