Rabbit Anti-Mouse Casp9 pAb

Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.

Rabbit Anti-Mouse Caspase 9 polyclonal antibody. Caspase 9 is one of the most important caspases among the caspase family members. Upon induction of apoptosis, cytochrome c released from mitochondria associates with procaspase 9/APAF1. The complex processes procaspase 9 into a large subunit (35 kDa or 17 kDa) and small (10 kDa) by self-cleavage at D315. Activated caspase 9 further cleaves other caspase members including caspase 3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. In addition to self-cleavage, procaspase 9 can also be cleaved in vivo by caspase 3 at D330. The process served as a positive feedback to amplify the apoptotic signal in caspase activation pathway.

Synthetic peptide surrounding amino acid 345 of mouse Caspase 9

Detects 45 kDa Procaspase 9, 35/37 kDa cleaved large fragment, and the 25 kDa fully cleaved Caspase 9.