Antibodies

Rabbit Anti-Mouse NFkB p65 Affinity Purified pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Mouse
Isotype IgG
Applikationen WB, ICC
Menge 100 ug
Host Rabbit
ArtNr CS-CPN101
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Western Blot Immunocytochemistry
Description
NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.
Formulation
100 ug (0.2 mg/ml) affinity purified rabbit anti-NFkB polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 25% glycerol, 0.5% BSA, 0.01% thimerosal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
The antibody detects primarily NFkappaB p65 in samples of human, mouse, and rat origins.
Immunogen
Synthetic peptide surrounding amino acid 274 of mouse NFkappaB p65
Purification
Affinity purified
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.2 mg/ml
Cross-Reactivity
Human, mouse, and rat
Application Notes
Western blotting (0.5-4 ug/ml), immunocytochemistry (10-20ug/ml). However, the optimal conditions should be determined individually. The antibody detects primarily NFkB p65 in samples of human, mouse, and rat origins. The optimal concentration should be determined by the user for each specific application.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 659,19 €
Preis: 659,19 €
lieferbar

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