Rabbit Anti-Mouse NFkB p65 Affinity Purified pAb

Western blotting (0.5-4 ug/ml), immunocytochemistry (10-20ug/ml). However, the optimal conditions should be determined individually. The antibody detects primarily NFkB p65 in samples of human, mouse, and rat origins. The optimal concentration should be determined by the user for each specific application.

NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50 has an isoform called p49/p52, and both proteins are derived from the amino-terminal of precursor protein p105 and p100. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), pro-inflammatory cytokines (IL-1 & TNF, etc.), and viral infection, IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.

Synthetic peptide surrounding amino acid 274 of mouse NFkappaB p65

The antibody detects primarily NFkappaB p65 in samples of human, mouse, and rat origins.