Rabbit Anti-Mouse Smac ABLO aa 222-237 (CT) Affinity Purified pAb

The inhibitor of apoptosis proteins (IAPs) regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes the inhibitory effect of IAPs on caspases was recently identified and designated Smac/DIABLO (1, 2). Smac/DIABLO is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates cytochrome c/Apaf-1/capase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases (3-6). Smac/DIABLO is expressed in a variety of human and mouse tissues (1, 2). This polyclonal antibody can be used for detection of Smac/DIABLO by Western blot at 0.5 to 1 ug/ml. mouse heart tissue lysate can be used as positive control and a 25 kDa band can be detected. It is human, mouse, and rat reactive. For research use only.