Antibodies

Mouse Anti-Rat Il-6 Clone 10E5 mAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Rat
Clon 10E5
Isotype IgG2b
Applikationen WB, FC, DB
Menge 0.5 mg
Host Mouse
ArtNr CS-CMI168
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western blot and dot blot analysis: working dilution of 0.1-1.0 ug/ml. Flow Cytometry for detection of intracellular IL-6 production: use 0.25 ug of this antibody to stain 106 rat peritoneal macrophages stimulated with LPS. The optimal concentration should be determined by the user for each specific application.
Description
Interleukin-6 (IL-6) is a cytokine that functions in inflammation and the maturation of B-cells. IL-6 is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through Interleukin-6 Receptor alpha (IL6R). The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states, including susceptibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.
Formulation
Liquid sterile filtered through a 0.22 um filter in PBS pH 7.2. Carrier and preservative free.
Specificity
Rat IL-6
Immunogen
Purified recombinant rat IL-6
Purification
Purified from ascites by Protein G affinity chromatography.
Storage and Stability
Store at 2-4C for up to one month. For long term use, store in aliquots at -20C. Avoid repeated freeze-thaw cycles.
Concentration
1.0 mg/mL
Purity
>95% as determined by SDS-PAGE
Menge: 0.5 mg
Lieferbar: In stock
Listenpreis: 555,15 €
Preis: 555,15 €
lieferbar

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Arbeitsanleitung

CS-CMI168.pdf