|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Antibody |
|
Specific against |
Rat |
|
Clon |
2G101H7 |
|
Isotype |
IgG1 |
|
Applikationen |
WB, FC, DB |
|
Menge |
0.5 mg |
|
Host |
Mouse |
|
ArtNr |
CS-CMI170 |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Applications |
Western blot and dot blot analysis: Use 0.1-1.0 ug of antibody. Flow Cytometry: Use 0.1-1.0 ug per 106 cells to detect intracellular IL-10 in stimulated rat peritoneal macrophages. The optimal antibody concentration should be determined for each specific application. |
| Description |
Mouse Anti-Rat IL-10 Clone 2G101H7 monoclonal antibody. IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. It has pleiotropic effects in immunoregulation and inflammation. IL-10 down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. It can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggest that IL-10 is an essential immunoregulator in the intestinal tract. |
| Formulation |
Purified immunoglobulin in phosphate buffered saline, pH 7.2, carrier and preservative free. Sterile filtered through a 0.22 micron filter. |
| Specificity |
Recognizes recombinant and native rat IL-10 |
| Immunogen |
Recombinant rat IL-10 |
| Purification |
Purified from ascites by Protein G affinity chromatography |
| Storage and Stability |
Store at -20C. Centrifuge vial prior to opening. Upon initial thawing, aliquot into small volumes and store at -20C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. |
| Concentration |
1.0 mg/ml |
| Purity |
>95% as determined by SDS-PAGE analysis |
