Antibodies

Rabbit Anti-Rat Casp9 Affinity Purified pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Rat
Isotype IgG
Applikationen WB, ICC
Menge 100 ug
Host Rabbit
ArtNr CS-CPC125
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot Immunocytochemistry Immunohistochemistry
Description
Rabbit Anti-Rat Active Caspase 9 Affinity Purified polyclonal antibody. Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with procaspase 9 (47 kDa) and APAF1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
Formulation
Liquid in PBS + 50% glycerol + 1% BSA + 0.02% thimerosal. Precaution: Thimerosal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Recognizes the cleaved rat Caspase 9 (37 kDa/17 kDa). It does not recognize full length Caspase 9 or any other caspases.
Purification
Biospecific affinity chromatography
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.2 mg/ml
Application Notes
For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunocytochemistry, use a working dilution of 10-20 ug/ml.For Immunohistochemistry, use a working dilution of 10-20 ug/ml.The optimal concentration should be determined by the user for each specific application.
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 863,97 €
Preis: 863,97 €
lieferbar

Angebot anfordern

Fragen zum Produkt?

Ihr Ansprechpartner:

Arne Pelz

sales@hoelzel.de

Arbeitsanleitung

CS-CPC125.pdf