Rabbit Anti-Rat Casp9 Affinity Purified pAb

For Western Blot, use a working dilution of 0.5-4 ug/ml.For Immunocytochemistry, use a working dilution of 10-20 ug/ml.For Immunohistochemistry, use a working dilution of 10-20 ug/ml.The optimal concentration should be determined by the user for each specific application.

Rabbit Anti-Rat Active Caspase 9 Affinity Purified polyclonal antibody. Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 9 is an important member of the caspase family. Upon induction of apoptosis, Cytochrome c released from mitochondria associates with procaspase 9 (47 kDa) and APAF1. The complex processes pro-caspase 9 into a large subunit (37 kDa/17 kDa) and a small subunit (10 kDa). Cleaved caspase 9 further processes other caspases including caspase 3 and caspase 6, to initiate a caspase cascade leading to apoptosis. The affinity purified antibody recognizing the active forms of caspase 9 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

Biospecific affinity chromatography

Recognizes the cleaved rat Caspase 9 (37 kDa/17 kDa). It does not recognize full length Caspase 9 or any other caspases.