Rabbit Anti-rat FasL Affinity Purified pAb

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Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Rat
Isotype IgG
Applikationen WB
Menge 100 ug
Host Rabbit
ArtNr CS-CPF107
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Western Blot
Description
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.
Formulation
Liquid in 100 ug (0.5 mg/ml) affinity purified rabbit anti-FasL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only.
Specificity
Detects ca.31 kDa FASLG in samples of mouse and rat origins. A ca.70 kDa band, possibly representing the FASLG and Fas complex can also be detected.
Immunogen
Synthetic peptide mapping to the C-terminus of rat FASLG
Purification
Affinity Purified
Storage and Stability
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles.
Concentration
0.5 mg/ml
Cross-Reactivity
Reacts with both mouse and rat.
Application Notes
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Detects ca.31 kDa FasL in samples of mouse and rat origins. A ca.70 kDa band, possible represents the FasL and Fas complex can also be detectedThe optimal concentration should be determined by the user for each specific application.

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Menge: 100 ug
Lieferbar: In stock
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