|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Antibody |
|
Specific against |
Rat |
|
Isotype |
IgG |
|
Applikationen |
WB |
|
Menge |
100 ug |
|
Host |
Rabbit |
|
ArtNr |
CS-CPF107 |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Applications |
Western Blot |
| Description |
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis. |
| Formulation |
Liquid in 100 ug (0.5 mg/ml) affinity purified rabbit anti-FasL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only. |
| Specificity |
Detects ca.31 kDa FASLG in samples of mouse and rat origins. A ca.70 kDa band, possibly representing the FASLG and Fas complex can also be detected. |
| Immunogen |
Synthetic peptide mapping to the C-terminus of rat FASLG |
| Purification |
Affinity Purified |
| Storage and Stability |
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles. |
| Concentration |
0.5 mg/ml |
| Cross-Reactivity |
Reacts with both mouse and rat. |
| Application Notes |
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Detects ca.31 kDa FasL in samples of mouse and rat origins. A ca.70 kDa band, possible represents the FasL and Fas complex can also be detectedThe optimal concentration should be determined by the user for each specific application. |
