Item no. |
CS-CPF107 |
Manufacturer |
Cell Sciences
|
Amount |
100 ug |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB |
Specific against |
Rat (Rattus norvegicus) |
Host |
Rabbit |
Isotype |
IgG |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Similar products |
Fasl, CD95-L, Tnfsf6, Apt1Lg1 |
Available |
|
Storage Conditions |
Store at -20C or in working aliquots at -80C for long term storage. Avoid repeated freeze-thaw cycles. |
Application Notes |
Western blotting (0.5-4 ug/ml). However, the optimal conditions should be determined individually. Detects ca.31 kDa FasL in samples of mouse and rat origins. A ca.70 kDa band, possible represents the FasL and Fas complex can also be detectedThe optimal concentration should be determined by the user for each specific application. |
Cross-Reactivity |
Reacts with both mouse and rat. |
Description |
Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis. |
Formulation |
Liquid in 100 ug (0.5 mg/ml) affinity purified rabbit anti-FasL polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. Precaution: Thiomersal is a poisonous and hazardous substance which should be handled by trained staff only. |
Immunogen |
Synthetic peptide mapping to the C-terminus of rat FASLG |
Purification |
Affinity Purified |
Specificity |
Detects ca.31 kDa FASLG in samples of mouse and rat origins. A ca.70 kDa band, possibly representing the FASLG and Fas complex can also be detected. |
Concentration |
0.5 mg/ml |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.