Antibodies

Goat Anti-Rat IL-3 beta Affinity Purified pAb

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Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Rat
Applikationen WB, ELISA
Menge 100 ug
Host Goat
ArtNr CS-CPI146
eClass 6.1 32160702
eClass 9.0 32160702
Lieferbar
Applications
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3beta (100 ng/ml), a concentration of 2.7-4.0 ug/ml of this antibody is required. Western Blot: To detect rat IL-3 beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-3 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. ELISA: Indirect: To detect rat IL-3 beta by indirect ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rat IL-3 beta. Sandwich: To detect rat IL-3 beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant rat IL-3 beta.
Description
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant rIL-3 beta. Anti-rIL-3 beta specific antibody was purified by affinity chromatography employing immobilized rIL-3 beta matrix.
Formulation
Lyophilized from PBS, pH 7.2
Specificity
Recognizes rat IL-3 beta
Immunogen
Recombinant rat IL-3 beta
Purification
Biospecific affinity chromatography
Reconstitution
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage and Stability
The lyophilized antibody is stable for at least 2 years from date of receipt at -20C. The reconstituted antibody is stable for at least two weeks at 2-8C. Frozen aliquots are stable for at least 6 months when stored at -20C. Avoid repeated freeze-thaw cycles.

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Menge: 100 ug
Lieferbar: In stock
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