Antibodies

Goat Anti-Rat Leptin pAb

Hersteller Cell Sciences
Kategorie
Typ Antibody
Specific against Rat
Applikationen WB, ELISA
Menge 100 ug
Host Goat
ArtNr CS-CPL111
Eclass 6.1 32160702
Eclass 9.0 32160702
Lieferbar
Applications
Western Blot ELISA
Description
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat Leptin. Anti-Rat Leptin specific antibody was purified by affinity chromatography employing immobilized Rat Leptin matrix.
Formulation
Lyophilized from PBS, pH 7.2
Specificity
Recognizes rat Leptin
Immunogen
Recombinant rat Leptin
Purification
Biospecific affinity chromatography
Reconstitution
Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the antibody to a concentration of 0.1-1.0 mg/ml.
Storage and Stability
Lyophilized antibody is stable for 2 years at -20C. Reconstituted antibody is stable for 2 weeks at 2-4C or for 6 months in working aliquots at -20C. Avoid repeated freeze-thaw cycles.
Application Notes
For Western Blot, when used at a concentration of 0.1-0.2 ug/ml in conjunction with compatible secondary reagents, the detection limit is 1.5-3.0 ng/lane under either reducing or non-reducing conditions.For Indirect ELISA, when used at a concentration of 0.5-2.0 ug/ml (assuming 100 ul/well antibody solution), in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/wellFor Sandwich ELISA, when used at a concentration of 0.5-2.0 ug/ml (assuming 100 ul/well antibody solution), in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well.The optimal concentration should be determined by the user for each specific application
Menge: 100 ug
Lieferbar: In stock
Listenpreis: 626,84 €
Preis: 626,84 €
lieferbar

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Ihr Ansprechpartner:

Arne Pelz

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Arbeitsanleitung

CS-CPL111.pdf