Goat Anti-Rat Leptin pAb

For Western Blot, when used at a concentration of 0.1-0.2 ug/ml in conjunction with compatible secondary reagents, the detection limit is 1.5-3.0 ng/lane under either reducing or non-reducing conditions.For Indirect ELISA, when used at a concentration of 0.5-2.0 ug/ml (assuming 100 ul/well antibody solution), in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/wellFor Sandwich ELISA, when used at a concentration of 0.5-2.0 ug/ml (assuming 100 ul/well antibody solution), in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well.The optimal concentration should be determined by the user for each specific application

Lyophilized from PBS, pH 7.2

Biospecific affinity chromatography

Lyophilized antibody is stable for 2 years at -20C. Reconstituted antibody is stable for 2 weeks at 2-4C or for 6 months in working aliquots at -20C. Avoid repeated freeze-thaw cycles.