|
Hersteller |
Cell Sciences
|
|
Kategorie |
|
|
Typ |
Antibody |
|
Specific against |
Rat |
|
Isotype |
IgG |
|
Applikationen |
WB |
|
Menge |
200 ug |
|
Host |
Rabbit |
|
ArtNr |
CS-CPM300 |
|
eClass 6.1 |
32160702 |
|
eClass 9.0 |
32160702 |
|
Lieferbar |
 |
| Applications |
Western blot, Immunohistochemistry, Neutralization |
| Description |
Macrophage Migration Inhibitory Factor (MIF) was one of the first cytokine activities to be discovered and was initially described as a T-cell derived factor that inhibits the random migration of macrophages. Recently, MIF was rediscovered as a pituitary hormone that acts as the counter-regulatory hormone for glucocorticoid action within the immune system. MIF was released from macrophages and T-cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production. MIF plays a critical role in the host control of inflammation and immunity. |
| Formulation |
Lyophilized with 0.05% sodium azide. Precaution: This product contains sodium azide: a poisonous and hazardous substance which should be handled by trained staff only. |
| Specificity |
Rat MIF |
| Immunogen |
Recombinant full-length rat MIF |
| Purification |
Protein A purified |
| Reconstitution |
Centrifuge vial prior to opening. Reconstitute to 1 mg/ml with 200 ul sterile distilled water. |
| Storage and Stability |
Store at 2-4C for short term storage or at -20C in small aliquots for long term storage. Avoid repeated freeze-thaw cycles. |
| Cross-Reactivity |
Reacts with both rat and mouse MIF. Cross-reactivity to MIF of other species has not been determined. |
| Application Notes |
For Western blot, use a working dilution of 1:2000. For Immunohistochemistry, use a working dilution of 1:500. The optimal concentration should be determined by the user for each specific application. |
