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Recombinant Human FLT3 Ligand HQ, Animal Free

ArtNr CS-CRF133A
Hersteller Cell Sciences
Menge 10 ug
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Purity > 98% as determined by SDS-PAGE analysis. Purified product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products FL
Lieferbar
Storage Conditions
Store lyophilized protein at -20C and reconstituted protein in working aliquots at -20C with a carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles .
Molecular Weight
20 and 22 kDa
Description
Recombinant human FLT3 Ligand contains 155 amino acids and a 14 amino acid Histidine-based tag for a total length of 169 amino acids. The predicted molecular weight of the recombinant FLT3 Ligand is 19.9 kDa, but the recombinant protein migrates as two bands with an apparent MW = 20 and 22 kDa in SDS-PAGE as a result of glycosylation.
Formulation
Lyophilized from a 0.2 um sterile filtered solution in 10 mM acetic acid, pH 5.0
Reconstitution
Centrifuge vial prior to opening . Add sterile PBS to the vial to fully solubilize the protein to a concentration of 10 ug/ml.
Specificity
1.11 x 106 units/mg
Biological Activity
Determined by Cell Proliferation Assay for the dose-dependent effects of serial dilutions of recombinant human FLT3 Ligand on AML5 cells. The ED50 is typically in the range of 0.9-1.4 ng/ml.
Endotoxin Level
< 0.005 ng/ug of FLT3 Ligand

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
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