Recombinant Human G-CSF

Hersteller Cell Sciences
Kategorie
Typ Proteins
Specific against Human
Menge 1.0 mg
Host CHO cells
ArtNr CS-CRG006C
Eclass 6.1 34160400
Eclass 9.0 42020190
Lieferbar
Description
Recombinant Human G-CSF is a single, glycosylated polypeptide chain containing 174 amino acids and purified by proprietary techniques. Granulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. G-CSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils.
Formulation
lyophilized from a concentrated (1 mg/ml) Phosphate- Buffered Saline, pH 7.4.
Reconstitution
Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.
Storage and Stability
Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.
GeneID
1440
Source
CHO cells
Molecular Weight
20.0 kDa
Purity
> 98.0% as determined by RP-HPLC and SDS-PAGE analyses
Specific Activity
1.27 x 108 IU/mg.
Endotoxin Level
< 0.1 ng/ug of G-CSF
Biological Activity
The ED50, calculated by the dose-dependant proliferation of mouse NFS-60 indicator cells (measured by 3H-thymidine uptake) is <0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg.
AA Sequence
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP.
Menge: 1.0 mg
Lieferbar: In stock
Listenpreis: 6.841,91 €
Rabatt: -10,0%
Preis: 6.157,72 €
Sie sparen: 684,19 €
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Arbeitsanleitung

CS-CRG006C.pdf