|
Hersteller |
Cell Sciences |
|
Kategorie |
|
|
Typ |
Proteins |
|
Specific against |
Human |
|
Menge |
1.0 mg |
|
Host |
CHO cells |
|
ArtNr |
CS-CRG006C |
|
eClass 6.1 |
34160400 |
|
eClass 9.0 |
42020190 |
|
Lieferbar |
 |
| Description |
Recombinant Human G-CSF is a single, glycosylated polypeptide chain containing 174 amino acids and purified by proprietary techniques. Granulocyte Colony Stimulating Factor is a growth factor and/or cytokine produced by the endothelium, macrophages and a number of other immune cells. G-CSF stimulates the bone marrow to produce granulocytes and also to stimulate the survival, proliferation, differentiation and function of neutrophil granulocyte progenator cells and mature neutrophils. |
| Formulation |
lyophilized from a concentrated (1 mg/ml) Phosphate- Buffered Saline, pH 7.4. |
| Reconstitution |
Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions. |
| Storage and Stability |
Store lyophilized protein at -20C to -80C. Reconstituted protein is stable for 1 week at 2-4C. For long term storage, aliquot and store at -20C to -80C with a carrier protein (0.1% HSA or BSA) as a stabilizer. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles. |
| GeneID |
1440 |
| Source |
CHO cells |
| Molecular Weight |
20.0 kDa |
| Purity |
> 98.0% as determined by RP-HPLC and SDS-PAGE analyses |
| Specific Activity |
1.27 x 108 IU/mg. |
| Endotoxin Level |
< 0.1 ng/ug of G-CSF |
| Biological Activity |
The ED50, calculated by the dose-dependant proliferation of mouse NFS-60 indicator cells (measured by 3H-thymidine uptake) is <0.07 ng/ml, corresponding to a Specific Activity of 1.27 x 108 IU/mg. |
| AA Sequence |
TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASHLQSF LEVSYRVLRH LAQP. |
