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Recombinant Human IFN-gamma HQ

ArtNr CS-CRI249A
Hersteller Cell Sciences
Menge 10 ug
Kategorie
Typ Proteins
Specific against Human (Homo sapiens)
Purity > 98% as determined by SDS-PAGE analysis. Purified product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Similar products IFN-gamma, IFG, IFI, interferon gamma, immune interferon
Lieferbar
Storage Conditions
Store lyophilized protein at -20C and reconstituted protein in working aliquots at -20C with a carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles .
Molecular Weight
25 and 27 kDa
Description
Recombinant human IFN-gamma contains 143 amino acid residues and a 13 amino acid Histidine-based tag for a total length of 156 amino acids. The predicted molecular weight of the recombinant IFN-gamma with His tag polypeptide is 16.6 kDa, but the recombinant protein migrates as two bands with an apparent MW = 25 and 27 kDa in SDS-PAGE as a result of glycosylation.
Formulation
Lyophilized from a 0.2 um sterile filtered solution in PBS, pH 7.4
Reconstitution
Centrifuge vial prior to opening . Add sterile PBS to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml.
Biological Activity
Determined by the dose-dependent cytostatic effects of serial dilutions of IFN-gamma in the presence of TNF-alpha, on HT-29 (human carcinoma) cells. The ED50 is typically below 5 ng/ml.
Endotoxin Level
< 0.005 ng/ug of IFN-gamma

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
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