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Recombinant Human IFN-gamma HQ

Recombinant Human IFN-gamma HQ

Item no.	CS-CRI249A
Manufacturer	Cell Sciences
Amount	10 ug
Category	Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines
Type	Proteins
Specific against	Human (Homo sapiens)
Purity	> 98% as determined by SDS-PAGE analysis. Purified product carries no pyrogenic or pro-inflammatory contaminants, as assayed with monocyte activation test using Human 10-plex Cytokine Assay measuring IL-6, TNF-alpha and IL-1beta induction.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Similar products	IFN-gamma, IFG, IFI, interferon gamma, immune interferon
Available
Storage Conditions	Store lyophilized protein at -20C and reconstituted protein in working aliquots at -20C with a carrier protein (0.1% HSA or BSA) as a stabilizer for long term storage. Please note that the addition of any carrier protein into this product may produce unwanted endotoxin. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles .
Molecular Weight	25 and 27 kDa
Description	Recombinant human IFN-gamma contains 143 amino acid residues and a 13 amino acid Histidine-based tag for a total length of 156 amino acids. The predicted molecular weight of the recombinant IFN-gamma with His tag polypeptide is 16.6 kDa, but the recombinant protein migrates as two bands with an apparent MW = 25 and 27 kDa in SDS-PAGE as a result of glycosylation.
Formulation	Lyophilized from a 0.2 um sterile filtered solution in PBS, pH 7.4
Reconstitution	Centrifuge vial prior to opening . Add sterile PBS to the vial to fully solubilize the protein to a concentration not less than 100 ug/ml.
Biological Activity	Determined by the dose-dependent cytostatic effects of serial dilutions of IFN-gamma in the presence of TNF-alpha, on HT-29 (human carcinoma) cells. The ED50 is typically below 5 ng/ml.
Endotoxin Level	< 0.005 ng/ug of IFN-gamma

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10 ug

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Delivery expected until 1/22/2026

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